5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Details-enriched characterization of cell populations. Morphological data from pictures can insert into the richness of flow cytometry facts. As an example, the figure displays an otherwise common apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in Just about every population to expose morphologically distinctive attributes. These insights could not have been gained from multiplex staining by yourself.
As samples are acquired on the Attune CytPix Flow Cytometer, the significant-pace brightfield digicam captures and merchants illustrations or photos of detected occasions at a charge approximately six,000 images for each next, according to the flow level and picture dimension. For bigger adaptability, Attune Cytometric Computer software helps you to change the image capture frequency as wanted and select a particular gate for image seize, if needed.
Mix acoustic focusing engineering with substantial-parameter Investigation for adaptable spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing having a superior-speed brightfield digital camera for simultaneous significant throughput flow cytometry and significant resolution brightfield imaging.
"A picture isn't really quite really worth one thousand dots, but it genuinely helps to see images. It is excellent to see photos that correlate with staining. One example is, AnnexinV-good cells which have been morphologically distinctive than balanced cells, or CD14+ monocytes that are bigger and more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is illuminating to view the amount of doublets slip by means of common singlet gates, and terrific to confirm the status of surprising double-constructive events in my Evaluation."
Optimizing gating system. Even robust handbook singlet gating is mistake-susceptible and continues to be a subjective determination stage in Virtually all flow cytometry assays. Imaging can be used to substantiate and adjust gates to incorporate only one cells of interest.
Our scientists will style and design panels for yourself as repeatedly you would like—cost-free, no merchandise acquire needed.
The Attune NxT cytometer more info is the reliable workhorse instrument your lab can rely on for consistent and trusted overall performance. The Attune CytPix model incorporates a significant-pace brightfield digicam and AI-driven graphic Assessment computer software, with current updates like equipment Discovering resources and an intuitive user interface, enabling enhanced knowledge precision, accuracy, and quicker insights into sample biology.
Probably most significantly, these events have cells of Obviously distinct phenotypes which could lead to incorrect conclusions regarding double positive occasions (specifically in uncommon populations).
AI-pushed automatic image Evaluation Along with the Attune Cytometric Program interprets event attributes into distinctive morphology parameters which can be coupled with standard fluorescence and scatter parameters to expose really reproducible populace changes and one of a kind subsets.
Software note: Automatic image Examination cuts down person-to-consumer variability in flow cytometry gating procedures
Created-in dimensionality reduction equipment greater resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate anticipated population resolution in lysed complete human blood. User can see activities from each cluster for Visible affirmation of cell phenotypes.
Versatile payment system from Agilent Economic Remedies assist you to receive the vital instrumentation you'll need for instant undertaking, or to expand your lab.
At the guts of each Attune Flow Cytometer lies our innovative acoustic concentrating technological know-how, which utilizes seem waves to meticulously align cells in the flow mobile. This impressive method surpasses conventional hydrodynamic procedures, resulting in more rapidly processing speeds, Fantastic facts high quality, and improved sample throughput.
As the mobile line had Formerly been analyzed about the Attune CytPix Flow Cytometer, the scientists went again to the pictures and were capable of document the microbial infection no less than five days earlier.
Agilent Value Assure ensures you at the very least ten years of instrument use within the date you buy. Usually, We'll credit score you Using the residual value of the technique toward an upgraded product.
In this article, we exhibit that by gating methods employing image-based quantitative parameters can distinguish interacting cells from coincident situations to a lot more correctly examine interacting cells.